How do I mark a sequence as circular or linear? How can I view the bases for mapped reads that extend beyond the end of a reference sequence? How are mappings to circular references handled when exporting to or importing from SAM/BAM files? How can I create a heat map for a specific list of genes? How can I change the order of groups being compared in the Differential Expression for RNA-seq tool? How can I identify differential expression between groups affected by more than one factor? How can I create a Metadata Table for my RNA-Seq experiment in the Workbench? Why do I get p-values of zero from Differential Expression Analysis? How can I check that a set of tracks are compatible?Ĭan I use the De Novo Assembly tool for my transcriptomic data? How can I specify the column order for stacked visualizations of OTU abundance tables? Why are there ambiguity codes and N's in the paired reads of my mapping? How do I remove contaminant reads from my read list? Why do I get contigs shorter than the minimum contig length I specified? QIAGEN CLC Genomics Workbench | This FAQ described why including all data in one de novo assembly is recommended. Why should a CLC de novo assembly not be run in stages? This is a common requirement for downstream applications such as RAST or MT-RAST.Ĭan sequences downloaded from NCBI be used as a track-based reference? QIAGEN CLC Genomics Workbench | This FAQ page addresses how you can export a fasta file that includes coverage information along with the sequence name. How can contig coverage be included in exported fasta headers? QIAGEN CLC Genomics Workbench | This FAQ provide background information regarding why it is often not appropriate to use a masked reference for mapping Should I use a masked reference when working with exome or amplicon data? #CLC RNA SEQ HOW TO#How to add additional information from the annotation tracks to my RNA-Seq results? QIAGEN CLC Genomics Workbench | This FAQ described when a variants is classified as homozygous or heterozygous How are the homozygous and heterozygous calls determined? How can I view trace data in the Workbench? How can I run a batch job with multiple libraries for each sample? Why is my destination vector not being accepted by the Gateway Cloning tool?Īre the tools of the CLC Workbenches or Servers multi-threaded? Why are my att sites not recognized by the Gateway Cloning tool? How can I analyze and visualize strand specific RNA-Seq data? How to import predesigned primers to a CLC Workbench? How to trim adapters from miRNA data sequenced on Illumina machine? How to add flanking sequence to variants in the CLC Genomics Workbench? What do Ns represent in the output of my de novo assembly? QIAGEN CLC Genomics Workbench | Described the different way of prediction amino acid changes when working with Stand-alone objects or Tracks How can I get amino acid prediction information for the variants in my data? QIAGEN CLC Genomics Workbench | How to get an estimate for memory requirements for de novo assembly in CLC Workbench How much memory does a de novo assembly take? Why do I see a grey box with text saying "Too much data for rendering" when I view a mapping? Why do I get the error message "Inconsistent input: The provided metadata does not describe the input data." in the Differential Expression for RNA-Seq wizard? How can I trim and assemble my forward and reverse Sanger sequence for each sample in batch using QIAGEN CLC Workbench? How to save the decorations on a phylogenetic tree? Which steps should I follow to perform a resequencing analysis in the CLC Genomics Workbench?
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